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Larry DeLucas, OD, PhD
High-Throughput Self-Interaction Chromatography: Applications to Protein Solubility and Stability
Director, Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, Birmingham, Alabama
2 Riverside Circle, Roanoke, VA 24016
The physical stability of a protein generally depends on suppression of aggregation. In either case, it is ultimately the protein-protein interactions in the specified solvent condition that dictates the process outcome. A novel technology, High-throughput Self-interaction Chromatography (HSC), is presented for measuring protein-protein interactions. The combination of the HSC technology and a balanced screen of different additives in solution are used to measure protein-protein interactions. The results are put into an artificial neural network program that predicts new solution conditions with novel additive combinations and concentrations. Examples demonstrating its utility for rapidly optimizing protein solubility and physical stability will be presented.
In addition, Dr. DeLucas will briefly describe a new concept that involves use of embryonic stem cells to express milligram quantities of membrane proteins in an essentially purified form. The presentation will conclude with a summary of a major protein crystallization initiative currently underway on the International Space Station.
*Please note the time change from 11:00 to 1:00.